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Image Search Results
Journal: Advanced therapeutics
Article Title: Inhibition of Collagenase Q1 of Bacillus cereus as a Novel Antivirulence Strategy for the Treatment of Skin-Wound Infections
doi: 10.1002/adtp.202100222
Figure Lengend Snippet: a,b) Quantification of Hyp release over time after treatment with different concentrations of a) B . cereus csn (0−100% v/v) and b) ColQ1 (0−500 × 10 −9 M). This graph contains data adapted from our previous publication. c) Confocal SHG Z-stack images of the COL structure in skin dermal region that was non-treated or treated with 300 × 10 −9 M ColQ1. d) B . cereus ColQ1 (300 × 10 −9 M) degraded the fibrillar COLs, immunostaining of non-treated and ColQ1 treated skin with COL antibodies (COL I, III, and V). COL: collagen, Hyp: hydroxyproline, B . cereus : Bacillus cereus , csn: culture supernatant, SHG: second harmonic generation. Data point represents mean value ± standard deviation ( n = 3). Scale bar: 100 μm for SHG images and immunostained images. Bright-field and DAPI images of the immunostained non-treated and ColQ1-treated tissue are shown in , Supporting Information.
Article Snippet: For immunostaining, tissue samples were stained with primary antibodies. (
Techniques: Immunostaining, Standard Deviation
Journal: Advanced therapeutics
Article Title: Inhibition of Collagenase Q1 of Bacillus cereus as a Novel Antivirulence Strategy for the Treatment of Skin-Wound Infections
doi: 10.1002/adtp.202100222
Figure Lengend Snippet: a) Chemical structures of compounds 1 and 2 and the calculated IC 50 value in the FRET-based ColQ1, ColH, and LasB inhibition assay. b) Effect of ColQ1 inhibitors on the cleavage of COL I after challenge with 50 ng of ColQ1. c,d) Effect of compounds 1 and 2 on B . cereus csn monitored by c) gelatin zymography. The gelatin-degradation assay was performed in the presence of inhibitors or the buffer control. Due to limited solubility in the reaction buffer, compound 2 could only be tested at 100 × 10 −6 m compared to 300 × 10 −6 m of compound 1 . d) Densitometric analysis of gelatin zymography shown in (c). Image analysis was performed with Image Studio Lite v5.2 software (Li-Cor Biosciences, USA). B . cereus : Bacillus cereus , csn: culture supernatant, COL: collagen, n.d.: not determined.
Article Snippet: For immunostaining, tissue samples were stained with primary antibodies. (
Techniques: Inhibition, Zymography, Degradation Assay, Solubility, Software
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: The Injectable Woven Bone-Like Hydrogel to Perform Alveolar Ridge Preservation With Adapted Remodeling Performance After Tooth Extraction
doi: 10.3389/fbioe.2020.00119
Figure Lengend Snippet: In vitro biological activity test of IWBLHs. (A) The cell toxicity result of extract of various IWBLHs. (B) The BMSCs adhesion results of IWBLHs. (C) BMSCs proliferation result of IWBLHs. (D) ALP and (E) OCN of BMSCs growing on various IWBLHs, the weight ratio indicated SA: ACP: mineralized collagen fibril ( n = 3, experiments performed in triplicate. The bars show the mean ± SD, * p < 0.05).
Article Snippet: At the predetermined time, the alkaline phosphatase (ALP) and osteocalcin (OCN) of cells were tested using the ALP assay kit (Beyotime Institute of Biotechnology, China) and
Techniques: In Vitro, Activity Assay